lambda zapii phage vector  (Agilent technologies)

Journal:

Article Title: Molecular Cloning of a Bacteroides caccae TonB-Linked Outer Membrane Protein Identified by an Inflammatory Bowel Disease Marker Antibody

doi: 10.1128/IAI.69.10.6044-6054.2001

Figure Lengend Snippet: Pep1 hybridization of a 2-kb AccI genomic segment of B. caccae. Three clones (5-2, 3-2, and 1-1) were isolated from a primary screen of a genomic B. caccae λ phage library. The clones were digested with AccI, electrophoresed, transferred to nitrocellulose membranes, and hybridized with the Pep1 or Pep2 oligonucleotide probe. (Left panel) Ethidium bromide-stained gel; (middle panel) membrane hybridization with Pep1 oligonucleotide; (right panel) membrane hybridization with Pep2 oligonucleotide.

Article Snippet: Genomic library of a B. caccae clinical isolate (p2Lc3) was constructed using lambda ZAPII λ phage vector (Stratagene, La Jolla, Calif.).

Techniques: Hybridization, Clone Assay, Isolation, Staining

Journal:

Article Title: Molecular Cloning of a Bacteroides caccae TonB-Linked Outer Membrane Protein Identified by an Inflammatory Bowel Disease Marker Antibody

doi: 10.1128/IAI.69.10.6044-6054.2001

Figure Lengend Snippet: Cloning of the ompW gene. A genomic λ phage library of p2Lc3 was constructed and screened using Pep1 and Pep2 probes. Southern analysis identified an authentic Pep1-positive genomic clone, designated 5-2 (Fig. ​(Fig.1),1), which contained an artifactual insert Tn10 sequence. The native ompW gene was isolated by genome walking using p2Lc3 genomic DNA as substrate. Sequence analysis of a 4,043-bp fragment revealed an open reading frame encoding 947 amino acids and an upstream AT-rich putative promoter locus.

Article Snippet: Genomic library of a B. caccae clinical isolate (p2Lc3) was constructed using lambda ZAPII λ phage vector (Stratagene, La Jolla, Calif.).

Techniques: Clone Assay, Construct, Sequencing, Isolation